Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024827_ez9n GGAGTGATCCCAGGCCAAAACAAGACACAGTG(+ATATAT)GATCCCCCTTATCCTCTGGGGAAATATTCCA NGG 10 8 17 8 24 0 100 5 10
    240607024826_sghs GGCCTGGGTATTTAGAAAGCAGAGGCACACGG(+ATATAT)GATCCCGATGACCATAGGCAGCACCGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607024825_j6e7 GAAGGCCCCTCGGTGCTGCCTATGGTCATCGG(+ATATAT)GATCCCGTGTGCCTCTGCTTTCTAAATACCC NGG 10 8 17 8 24 0 100 5 10
    240607024822_ozty CAAACTTGTAAGATACCATTCACCTTTCCCAG(+ATATAT)GTACCTGCTACCAAGTCACCTGGCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240607024821_e08a CAGAAGGTTGCTGCCAGGTGACTTGGTAGCAG(+ATATAT)GTACCTGGGAAAGGTGAATGGTATCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240607024819_zd15 CTCCAGAAAGATGGCCACAGCCCAGCTCCCTG(+ATATAT)GTACCAAGCTGTCATCCCTAAACTTGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240607024818_9e7t GACAAAGGGCCAAGTTTAGGGATGACAGCTTG(+ATATAT)GTACCAGGGAGCTGGGCTGTGGCCATCTTTC NGG 10 8 17 8 24 0 100 5 10
    240607024816_4gqm GAGCCGCGTGACTGTACGTGTTGAAGGGGGCG(+ATATAT)GTACCTGTTCACAGCACAAGTTTATGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607024815_68x8 CAGAGCTCAACATAAACTTGTGCTGTGAACAG(+ATATAT)GTACCGCCCCCTTCAACACGTACAGTCACGC NGG 10 8 17 8 24 0 100 5 10
    240607024813_yluq ACTCCCACACTCCACGTACTTGTTCGGAGCAG(+ATATAT)GTACCACTGACTACAAAGTCATCAGTTATTG NGG 10 8 17 8 24 0 100 5 10