Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024927_7qna CATAGATAGGCCCAGGGAGTGCTTTCAGACAG(+ATATAT)GTACCAGATAAGGTGTGTTAAGCTGTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607024926_bvg0 ATTGTTAATGTGCCTTGCAACTGGGAGTCTAG(+ATATAT)GTACCTAATGAAACTTTGTGACTTTAGCACA NGG 10 8 17 8 24 0 100 5 10
    240607024925_yl3b GAACTTGTGCTAAAGTCACAAAGTTTCATTAG(+ATATAT)GTACCTAGACTCCCAGTTGCAAGGCACATTA NGG 10 8 17 8 24 0 100 5 10
    240607024923_f68o GCCTGGCCTGGGTGGGCCTGTGCTGTGCCTTG(+ATATAT)GTACCATCTTCCCTCGATTTTCCTTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240607024922_tir6 GAAGGAGGGTCAAGGAAAATCGAGGGAAGATG(+ATATAT)GTACCAAGGCACAGCACAGGCCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607024921_skx3 CATGCATATTATTTATTTCTGTGCAAACTGTG(+ATATAT)GATCCGTAACCAAGGTGAAGAAAGTTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607024919_e8d0 CAGAAATCCCAACTTTCTTCACCTTGGTTACG(+ATATAT)GATCCACAGTTTGCACAGAAATAAATAATAT NGG 10 8 17 8 24 0 100 5 10
    240607024918_k0zd CTTCACATAGCCACATCATAGACGTTTTTAGG(+ATATAT)GATCCCTGCTCTCCTAAATTGCTCCCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240607024916_9so6 TACTGACTCGGGGAGCAATTTAGGAGAGCAGG(+ATATAT)GATCCCTAAAAACGTCTATGATGTGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240607024914_i9q0 CCTAACTCTGAGCAAGTGACTTCTGCAAAAGG(+ATATAT)GTACCCCGAAGGAAGCCATGTGGGCATCCTG NGG 10 8 17 8 24 0 100 5 10