Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024957_qktf TGTAGACATGGGTTCTTTCCTTCCACGCTGTG(+ATATAT)GATCCTGGGGATCAAGCACATTATCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240607024956_jtmr TTGGTTTGGATGGAAGATGAGCAGCCTGGTGG(+ATATAT)GATCCTTTGGAGGGTAAGATGGTGATGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607024954_a27x ACTCTAAAACATCACCATCTTACCCTCCAAAG(+ATATAT)GATCCCACCAGGCTGCTCATCTTCCATCCAA NGG 10 8 17 8 24 0 100 5 10
    240607024952_tbbi TAAAGTGATGGAGGGCAACGGAGGGTGATGGG(+ATATAT)GATCCTGAAGCCAGGAGTCAGAAATCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607024951_yhbp ATGACACCAAGATTTCTGACTCCTGGCTTCAG(+ATATAT)GATCCCCATCACCCTCCGTTGCCCTCCATCA NGG 10 8 17 8 24 0 100 5 10
    240607024949_yb4t CAGCTGTAACCTTCAGTCTTCCACATAGCCTG(+ATATAT)GTACCTACTGACTCGATATTGACCACTGACA NGG 10 8 17 8 24 0 100 5 10
    240607024948_z9se AACCATGTCAGTGGTCAATATCGAGTCAGTAG(+ATATAT)GTACCAGGCTATGTGGAAGACTGAAGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607024947_3nt3 ATCGAGTGCGCAGGTGCACCACATGCATGCAG(+ATATAT)GTACCCAGGAGGATGTTAGATTCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240607024945_cgh9 CTCCAGTTCCAAGGAATCTAACATCCTCCTGG(+ATATAT)GTACCTGCATGCATGTGGTGCACCTGCGCAC NGG 10 8 17 8 24 0 100 5 10
    240607024944_cb4v TCGTCCACAGGACAGATGAGAAAATAGCTTTG(+ATATAT)GTACCTGTCCACCACCGTCCCCGCCGTCACA NGG 10 8 17 8 24 0 100 5 10