Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024759_vpxu ACAAAAGAACATAGACAGTAATTGTTACTTTG(+ATATAT)GATCCATTAATTTTATTAGTGCTTTATTACT NGG 10 8 17 8 24 0 100 5 10
    240607024757_g3ei TCTTTCTAAGGCAAGATCAGCCATCACATGTG(+ATATAT)GTACCTTCCATGCATTCTGAACTCTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607024756_x3pi GAGAGAGAGAGAGAGTTCAGAATGCATGGAAG(+ATATAT)GTACCACATGTGATGGCTGATCTTGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240607024755_xjan TCATGCCCTGCCCCAAAGTAAAGGGTAAGTAG(+ATATAT)GTACCATTTAGTGAAGGCCAGTATCATTTCA NGG 10 8 17 8 24 0 100 5 10
    240607024754_b20p AACAGTGAAATGATACTGGCCTTCACTAAATG(+ATATAT)GTACCTACTTACCCTTTACTTTGGGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607024752_zgc9 AGTTAAGCTTGCACATTTGGATAGGAAGGTGG(+ATATAT)GTACCCCGCTCCAGCAAGATCGTTTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240607024750_y4ax GCTTGTGCATAAAACGATCTTGCTGGAGCGGG(+ATATAT)GTACCCACCTTCCTATCCAAATGTGCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240607024749_ccmz AGGTAGAGCCCTGAGAGTTCCCAGCAGAGTGG(+ATATAT)GATCCAGACTTTGCACAGAAATGATCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607024748_jvip GGTGACCTGAGATCATTTCTGTGCAAAGTCTG(+ATATAT)GATCCCACTCTGCTGGGAACTCTCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607024746_ddi6 CCGGCCTCCTCCCCTCACACACTCTGGACTTG(+ATATAT)GTACCGAATAATCCCGTGTGGTCCTAGCTGA NGG 10 8 17 8 24 0 100 5 10