Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024717_zsjt CAAAATCCTGAATACTATAATATGATAACCAG(+ATATAT)GATCCTAAAATTTGTCCTAAAATTTTGACTT NGG 10 8 17 8 24 0 100 5 10
    240607024716_op6q TTTTAATAGAGAAACAGGACACTGATTTTGAG(+ATATAT)GATCCTGACTCCAAAGCTAATGTCCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607024714_xhls AGACTGAAGAAGGACATTAGCTTTGGAGTCAG(+ATATAT)GATCCTCAAAATCAGTGTCCTGTTTCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240607024713_y506 ACCAAACTCTGAAGGAAACACTGCTGAGGAAG(+ATATAT)GTACCCTAGTGGATAAGGTTAGATTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607024711_kn0u CAAGGCCCTGAAATCTAACCTTATCCACTAGG(+ATATAT)GTACCTTCCTCAGCAGTGTTTCCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607024710_5qk2 AAGCCATTTTGTTGAGGGAGGGGCTTTTCCTG(+ATATAT)GTACCTATCTCCTAAAGGGGTTACTTTCGAA NGG 10 8 17 8 24 0 100 5 10
    240607024708_ck34 GTGAATTCGAAAGTAACCCCTTTAGGAGATAG(+ATATAT)GTACCAGGAAAAGCCCCTCCCTCAACAAAAT NGG 10 8 17 8 24 0 100 5 10
    240607024707_mmh7 GTGAATTCGAAAGTAACCCCTTTAGGAGATAG(+ATATAT)GTACCAGGAAAAGCCCCTCCCTCAACAAAAT NGG 10 8 17 8 24 0 100 5 10
    240607024705_yjp5 AAGCCATTTTGTTGAGGGAGGGGCTTTTCCTG(+ATATAT)GTACCTATCTCCTAAAGGGGTTACTTTCGAA NGG 10 8 17 8 24 0 100 5 10
    240607024704_fte4 CAAGGCCCTGAAATCTAACCTTATCCACTAGG(+ATATAT)GTACCTTCCTCAGCAGTGTTTCCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10