Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024745_t6mb GTGCTTCAGCTAGGACCACACGGGATTATTCG(+ATATAT)GTACCAAGTCCAGAGTGTGTGAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607024743_9kby GTTACTTTAAGTCCTGAGATGCAGGTTCCCTG(+ATATAT)GTACCATTGAGTTGCTGCTACTAAAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607024742_1v2z GCATGTGAGCTTTTAGTAGCAGCAACTCAATG(+ATATAT)GTACCAGGGAACCTGCATCTCAGGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240607024741_m39u AGATACAGCTGTCCCGTGGAGCAGCAGGCATG(+ATATAT)GATCCCCTCCATCCTTGGGCTTCCCGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607024739_ir3b CGCAGGGGCCCGGGAAGCCCAAGGATGGAGGG(+ATATAT)GATCCATGCCTGCTGCTCCACGGGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607024738_qvtx GCCTATTTTGTGATGTGATGACCTTCAAATTG(+ATATAT)GATCCCGTGGCTTGTCCTCTCTGAAGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240607024736_f8jh GGGCTCCGGCCTTCAGAGAGGACAAGCCACGG(+ATATAT)GATCCAATTTGAAGGTCATCACATCACAAAA NGG 10 8 17 8 24 0 100 5 10
    240607024734_8awq AGGACCCACTGACTAGCAAATAGTAGGGTCCG(+ATATAT)GATCCGGTTCTAATCTGGGGAAGGGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607024732_lf7m ACAAACTGAGCCCCTTCCCCAGATTAGAACCG(+ATATAT)GATCCGGACCCTACTATTTGCTAGTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607024731_apnk ATCTAGTCATTCCAGACAGCTTTCTGTATGTG(+ATATAT)GATCCTGGCTTTGGATCAAAACCACTTCTGC NGG 10 8 17 8 24 0 100 5 10