Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025222_op8e CAGCCCATGTGTGTTGGAAAGCACATTCCGTG(+ATATAT)GTACCAGAGACAGTTTGCAGCCTTGCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607025220_zgsh GTTCCTCCACAGATGTGCTAAAGTCCAAAGAG(+ATATAT)GATCCATTTGGAAGGAGTGGCTTGGCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240607025219_gcx9 CACGGCCCCGGCCAAGCCACTCCTTCCAAATG(+ATATAT)GATCCTCTTTGGACTTTAGCACATCTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607025218_r3sh GAGTGGGTAGTTGTGGACTTGTTAACAGTAAG(+ATATAT)GTACCTTTGAAGTATGCAGATGGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240607025217_lwn1 CACTGGGTCACCTCCATCTGCATACTTCAAAG(+ATATAT)GTACCTTACTGTTAACAAGTCCACAACTACC NGG 10 8 17 8 24 0 100 5 10
    240607025216_yhx4 GACCGAGCTATGCATGGAGGAGGAGGCTGTTG(+ATATAT)GTACCATACTGAATTTCCACTGGACCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240607025215_fmnk TGACTTTAGAGGTCCAGTGGAAATTCAGTATG(+ATATAT)GTACCAACAGCCTCCTCCTCCATGCATAGCT NGG 10 8 17 8 24 0 100 5 10
    240607025213_yjb0 GTGGGCATTCATAGTCTAAATCCTCTAGGGAG(+ATATAT)GATCCTCTTGCCATCTGCCCTCCTCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240607025212_cbki CCTGGGCCACTGAGGAGGGCAGATGGCAAGAG(+ATATAT)GATCCTCCCTAGAGGATTTAGACTATGAATG NGG 10 8 17 8 24 0 100 5 10
    240607025210_jc76 GGCAGGCAGATGAGAAACCAGTTGGACCTGTG(+ATATAT)GATCCCAGTGGTTCCCGTGAGTGGATTTTTA NGG 10 8 17 8 24 0 100 5 10