Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025322_adta TATCTATGCCCAGAGTTTTCTCTGCAATCAGG(+ATATAT)GTACCTGTGCATTAGCCACAGTATCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240607025320_hb0x AAGGTACTACCGGCTATAGCCACAGGGATCCG(+ATATAT)GTACCATTGAGATCCCTTCATAAAATATGGA NGG 10 8 17 8 24 0 100 5 10
    240607025319_wcat CTTGCTCCATATTTTATGAAGGGATCTCAATG(+ATATAT)GTACCGGATCCCTGTGGCTATAGCCGGTAGT NGG 10 8 17 8 24 0 100 5 10
    240607025317_oclw TGCTCCCGCCTATCTTGCTCCTTAACACTGGG(+ATATAT)GATCCGATAGAGAAATGACACAAGGTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607025316_dsfx ACACACTGAAACCTTGTGTCATTTCTCTATCG(+ATATAT)GATCCCCAGTGTTAAGGAGCAAGATAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240607025314_ueo0 GCTCCTAAGGTACTACCGGCTATAGCCACAGG(+ATATAT)GATCCGGTACCATTGAGATCCCTTCATAAAA NGG 10 8 17 8 24 0 100 5 10
    240607025313_80pw CCATATTTTATGAAGGGATCTCAATGGTACCG(+ATATAT)GATCCCTGTGGCTATAGCCGGTAGTACCTTA NGG 10 8 17 8 24 0 100 5 10
    240607025312_q5im GGAGCCACAAGGAGTGGGCTTCATGCATTATG(+ATATAT)GATCCCGACCCCCGCCACTGTGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240607025310_levh TCCTGGAGCAAGCCCACAGTGGCGGGGGTCGG(+ATATAT)GATCCATAATGCATGAAGCCCACTCCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240607025307_92ga CCAGCAAGAAGGGGGAGCAGGGGACGAGGGGG(+ATATAT)GTACCTATCCTTCCTGAACGGACAATGGGGG NGG 10 8 17 8 24 0 100 5 10