Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025418_4d3h CTCGTTAGTCCTTCACAGACAATCAGTATCTG(+ATATAT)GATCCTAGTGAATGTAGCATCATTGACTTTA NGG 10 8 17 8 24 0 100 5 10
    240607025417_py53 TTGAGTCAAACCATGGTACTTGAATCATTGAG(+ATATAT)GATCCAAGTCATGATTTGAATTCTGTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240607025416_qatr AAAAATGGCAACAGAATTCAAATCATGACTTG(+ATATAT)GATCCTCAATGATTCAAGTACCATGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607025414_qzhv GCTTTCAATCATCTGTAACTCCAGTTCCAATG(+ATATAT)GATCCAACACCTTATAGCCTCTGGTACACAT NGG 10 8 17 8 24 0 100 5 10
    240607025413_7w6u TATGCATGTGTACCAGAGGCTATAAGGTGTTG(+ATATAT)GATCCATTGGAACTGGAGTTACAGATGATTG NGG 10 8 17 8 24 0 100 5 10
    240607025412_drkc CATACAGAGGGATACCACTGCTGTTGGGTCTG(+ATATAT)GTACCAGACACTCAGGCCCTTCATCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607025411_5976 ACGGAGAAGAAGATGAAGGGCCTGAGTGTCTG(+ATATAT)GTACCAGACCCAACAGCAGTGGTATCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607025410_dbu7 AAAATCCATGCTGCAGGGTAACCCTGTAAGTG(+ATATAT)GATCCACTGTGGAAACATTTTGTACATCACA NGG 10 8 17 8 24 0 100 5 10
    240607025408_evks GATACTGTGATGTACAAAATGTTTCCACAGTG(+ATATAT)GATCCACTTACAGGGTTACCCTGCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240607025407_3jxy GACATAAACTGGATATTGTTTCAATAGACAGG(+ATATAT)GATCCCATTCCCCAAACCACCTGGTTGCTAA NGG 10 8 17 8 24 0 100 5 10