Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025514_eaxx TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+ATATAT)GTACCATAAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240607025513_x3z0 AAAAGGATATACCTTCTTCTCAGCACCTTATG(+ATATAT)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240607025511_813m TTCCTCTCAAGTGCTGGGATTAATGCAATGGG(+ATATAT)GTACCAGGACTGGCAATAAAATTACCTACTT NGG 10 8 17 8 24 0 100 5 10
    240607025509_qezc CTTAGAAGTAGGTAATTTTATTGCCAGTCCTG(+ATATAT)GTACCCCATTGCATTAATCCCAGCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607025508_2scu TGGGAAGACCAGCCCCCACCCAGAGAATATTG(+ATATAT)GATCCTTGGATAAAAATCACAGACACAATGC NGG 10 8 17 8 24 0 100 5 10
    240607025506_tn9l ATTGAGCATTGTGTCTGTGATTTTTATCCAAG(+ATATAT)GATCCAATATTCTCTGGGTGGGGGCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607025504_955j GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+ATATAT)GATCCCCGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607025502_bb0m GGATGGACCATCTAGAGACTGCCATATCCGGG(+ATATAT)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607025500_b6hc TTGTGGATATATGCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607025458_n0dr TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10