Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607025351_zow5 AGTCAATCAAGACCACTTAAGGAAAAACTTGG(+ATATAT)GATCCAGGCATGGTAGCACATGCCTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607025349_u1ur GGAACTTTTCAGAAGATGCTTAGTGAAAGGGG(+ATATAT)GATCCGTTCAAATAGATGTCTGGTCCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607025348_a3ec ACCCTTGCAGGGACCAGACATCTATTTGAACG(+ATATAT)GATCCCCCTTTCACTAAGCATCTTCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607025346_50rk ACATTCTACCCTTTGGGTTCTAAGATTCCAGG(+ATATAT)GTACCAATAGCCATGCATGACAGCTGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607025344_vywc TGGTATGGCTCAGCTGTCATGCATGGCTATTG(+ATATAT)GTACCCTGGAATCTTAGAACCCAAAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240607025343_ag01 CTTGTGGGAGAGTTTTCGCTTTCCAGCATGTG(+ATATAT)GTACCAGGAATAGCATCAGGTTGTCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607025341_ic7e CTGCTGAGCCTGACAACCTGATGCTATTCCTG(+ATATAT)GTACCACATGCTGGAAAGCGAAAACTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607025340_di2x ACAAATAACTCATATGCAAAGCCAATTCGCAG(+ATATAT)GATCCTGGAAACGTATCAGGATACTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240607025339_q83c GCTTTTGAGATAGTATCCTGATACGTTTCCAG(+ATATAT)GATCCTGCGAATTGGCTTTGCATATGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240607025337_9jl3 GCCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10