Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024550_jdsz GAAAACCTTTGACTCTACCATGGCTGGCCCTG(+ATATAT)GATCCTCTTGTTGTAGGGGGTCTGGCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607024549_mi9o GCACAGCTGCCAAGGCTTAGAAGGAGAAGCTG(+ATATAT)GTACCGGCCTCTTCTTCAAAAGCAGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607024547_0a3u CAGCACCCTGGCTGCTTTTGAAGAAGAGGCCG(+ATATAT)GTACCAGCTTCTCCTTCTAAGCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607024545_svl0 GACATTTGACAAGTGTCCAATTTCTCCAAAGG(+ATATAT)GTACCTTAAGATTCTCAAAAAAAAAGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240607024544_erew TTTGAATTTACTTTTTTTTTGAGAATCTTAAG(+ATATAT)GTACCCTTTGGAGAAATTGGACACTTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240607024543_uldy ATTAATGTGGTTAAATCCAAAGTCTGAGGTAG(+ATATAT)GTACCTGAGCCTTGTTATTTTGGAAATTCAA NGG 10 8 17 8 24 0 100 5 10
    240607024541_8q69 TGAATTTGAATTTCCAAAATAACAAGGCTCAG(+ATATAT)GTACCTACCTCAGACTTTGGATTTAACCACA NGG 10 8 17 8 24 0 100 5 10
    240607024540_eueg TGAGTGCTTGAGGAGGCCAGAAGAGGGTGTTG(+ATATAT)GATCCCCTGGAGCTGAAGTAATGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240607024538_jchw GCTTACAATGGCCCATTACTTCAGCTCCAGGG(+ATATAT)GATCCAACACCCTCTTCTGGCCTCCTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240607024537_v0wc AGTCCCAATTTCAGGGTGGCACAAATTGTATG(+ATATAT)GATCCAGCTCTTGGATCACACAATCGCAAAG NGG 10 8 17 8 24 0 100 5 10