Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024522_mho0 CAGGCATAGTTAAGCAATCCTGGTCACGATGG(+ATATAT)GTACCTTTACTTTTGACATGAAAGCTTTCGA NGG 10 8 17 8 24 0 100 5 10
    240607024521_u9q9 GTTTTGGTCAAGCTGAGCTAGTCTGAGGTATG(+ATATAT)GTACCTCAGAATTACACAATAGCACCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240607024520_jjxn AGAGGGAGAGGGTGCTATTGTGTAATTCTGAG(+ATATAT)GTACCATACCTCAGACTAGCTCAGCTTGACC NGG 10 8 17 8 24 0 100 5 10
    240607024519_tnpq TGACTTCATTGAGTGTTCTCAGAAGAGCGCTG(+ATATAT)GTACCTTTTATAAAAGATTGTGGATCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607024517_svx6 TCAATCTGAAGATCCACAATCTTTTATAAAAG(+ATATAT)GTACCAGCGCTCTTCTGAGAACACTCAATGA NGG 10 8 17 8 24 0 100 5 10
    240607024516_logf CCTGTGTGATGTCAAATCCTCAGCCTGATCTG(+ATATAT)GTACCTTTCCTCTTTCCCACCTGCTGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607024515_e2j9 AGGGTGGCCTCAGCAGGTGGGAAAGAGGAAAG(+ATATAT)GTACCAGATCAGGCTGAGGATTTGACATCAC NGG 10 8 17 8 24 0 100 5 10
    240607024514_ebof AATGATGGGTACATGAGACTTGATTACAGATG(+ATATAT)GATCCCTTAACTCTGTGTTCATGTGATTCTG NGG 10 8 17 8 24 0 100 5 10
    240607024512_s1xx GTACACAGAATCACATGAACACAGAGTTAAGG(+ATATAT)GATCCATCTGTAATCAAGTCTCATGTACCCA NGG 10 8 17 8 24 0 100 5 10
    240607024511_kid2 CAGGCCTGACAAGCGGTAGAATCACCAGCTAG(+ATATAT)GTACCCAATCCACAATGAGGAAACCTTTGTA NGG 10 8 17 8 24 0 100 5 10