Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024439_k70j CTTGGGGGTAGCTGATATGGAACTTGATCAGG(+ATATAT)GATCCAAGGCTAAACACCTAGCCTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240607024437_koaq GTAGCTCTCAGTGGGACCTGTGGCTGTCCATG(+ATATAT)GTACCCATGGCAATTGGCCCAGCCTGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240607024435_6q5a CTGACATCCACAGGCTGGGCCAATTGCCATGG(+ATATAT)GTACCATGGACAGCCACAGGTCCCACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607024434_sz17 TGTACGCGCGGCACAAAGGTCTGCACTCAGTG(+ATATAT)GTACCTGCGGGTTGCCCAGCAGGGTTCACAC NGG 10 8 17 8 24 0 100 5 10
    240607024432_06q3 CCCGTGTGTGAACCCTGCTGGGCAACCCGCAG(+ATATAT)GTACCACTGAGTGCAGACCTTTGTGCCGCGC NGG 10 8 17 8 24 0 100 5 10
    240607024430_y5dj CGCACCCGCTCCCGCGTGGTGGCTCTTAGCGG(+ATATAT)GTACCCTGGGAGCGCCTGCCTCACGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607024428_m42o AGAAGGGCTCACGTGAGGCAGGCGCTCCCAGG(+ATATAT)GTACCCGCTAAGAGCCACCACGCGGGAGCGG NGG 10 8 17 8 24 0 100 5 10
    240607024426_rcvv AGGTCCCAGCATCAATGCCCAAGGCTCCCAAG(+ATATAT)GATCCTGGCTGGGTAAGGCACGGAGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607024424_kqnd AGAAACCCAAACTCCGTGCCTTACCCAGCCAG(+ATATAT)GATCCTTGGGAGCCTTGGGCATTGATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607024423_5a41 TCAGAAGGCACTGGAGAAGGCAGCGGGCAGAG(+ATATAT)GATCCTGGTAGGAATGTGGGACCCTGGGCTG NGG 10 8 17 8 24 0 100 5 10