Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024349_894d TCAGATCCTTGCAGCAGCCTTTCCAGGGGCAG(+ATATAT)GTACCCTAACCCTGTCCCCAGGCTCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607024348_sr5v AGAGCAATGTCTGATTTCACAACTTTTTCACG(+ATATAT)GATCCCTGAGACCACGATTAAAATCAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607024347_0r7q TTTTAAAACCTGATTTTAATCGTGGTCTCAGG(+ATATAT)GATCCGTGAAAAAGTTGTGAAATCAGACATT NGG 10 8 17 8 24 0 100 5 10
    240607024345_dbw2 AGTTCTGTGGCTGTGCTCATCTGTGTCATGGG(+ATATAT)GATCCCCCACAACCTTTGACTTTCTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607024343_mawr AAATGCTCCAAAGAAAGTCAAAGGTTGTGGGG(+ATATAT)GATCCCCATGACACAGATGAGCACAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240607024341_sj4i ATGGACCAAGGGTTTCATGGGGCAGAAGGAGG(+ATATAT)GTACCCAGAATTCCCAGCCCTCATCTGGTCC NGG 10 8 17 8 24 0 100 5 10
    240607024340_0itp GGCTGGGACCAGATGAGGGCTGGGAATTCTGG(+ATATAT)GTACCCTCCTTCTGCCCCATGAAACCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240607024338_o59y CCATTGCTTCCCCTTATAAGATGTCCCTGCAG(+ATATAT)GTACCAGAGCCAGTGACAGCACACACACAGG NGG 10 8 17 8 24 0 100 5 10
    240607024337_xe91 AGGGTCCTGTGTGTGTGCTGTCACTGGCTCTG(+ATATAT)GTACCTGCAGGGACATCTTATAAGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607024336_ihy6 GGCGTACCCAGGCCAGCACCAGAGGGCGGCAG(+ATATAT)GTACCCCAGGGAGTGGCTAGGGAGGTTGGTC NGG 10 8 17 8 24 0 100 5 10