Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024301_o18c GTCTCATGGCATAAGCATGCTGTGGGGAATGG(+ATATAT)GTACCCTGGGGCACAGCAGGGTCATTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240607024300_i1lt CTTGCATGTGGAGACCAGGGGTTGACATCAAG(+ATATAT)GATCCTCCTTAATCATTTATCCACTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607024258_botw TTTAAATGGTGAGTGGATAAATGATTAAGGAG(+ATATAT)GATCCTTGATGTCAACCCCTGGTCTCCACAT NGG 10 8 17 8 24 0 100 5 10
    240607024256_ui2y GGCACTGACCTGCCCCATTTCCTGATCCTTGG(+ATATAT)GATCCTAGAAACGCATACAAGGAGGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240607024255_ti87 AGGCCACTGTCCCTCCTTGTATGCGTTTCTAG(+ATATAT)GATCCCAAGGATCAGGAAATGGGGCAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607024254_veux TCCTGTTCAAATGTACCTCAGTGGAGAGAGAG(+ATATAT)GATCCTCGCTTCTACACTGTGTGGCCTGACG NGG 10 8 17 8 24 0 100 5 10
    240607024253_75wg ACTTCCGTCAGGCCACACAGTGTAGAAGCGAG(+ATATAT)GATCCTCTCTCTCCACTGAGGTACATTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607024252_sskp CCTGTAGAAGGCAAATGAGACACTGATTAGTG(+ATATAT)GATCCCTGCAGAACTGGGTCCTAGATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607024251_3i9n AAGCCTTTGGATCTAGGACCCAGTTCTGCAGG(+ATATAT)GATCCACTAATCAGTGTCTCATTTGCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607024250_uimt GCTGTATCAGAGGCAGCTTTCATCTTAGCATG(+ATATAT)GTACCCAGAAAAGGTTGGACATCAGCAAACA NGG 10 8 17 8 24 0 100 5 10