Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024206_27sy AGATTCAAATTCTTCCTCACCTGGTCCTGGAG(+ATATAT)GATCCCTGCCTGAGAACACACTCAGCTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607024205_u7jv TCAAAACACAAGGTCGGGGGCAATAGGAGATG(+ATATAT)GTACCTGATGCCAGTCTCATCTCCAACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607024204_1jd1 ACATGTGCAGTTGGAGATGAGACTGGCATCAG(+ATATAT)GTACCATCTCCTATTGCCCCCGACCTTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607024203_bfgu CAAGGTAGAGTGTTCATACCTGGGGAAAGCAG(+ATATAT)GTACCCTCTGGGTCTACATGGGGAGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607024201_y6rg GCTCTCTCTCTCTCCCCATGTAGACCCAGAGG(+ATATAT)GTACCTGCTTTCCCCAGGTATGAACACTCTA NGG 10 8 17 8 24 0 100 5 10
    240607024200_2q6p CACAGGCTGCTCCCCACCTCAGTTCTATAAAG(+ATATAT)GATCCAGACAGGAGAGGCCAAGAGAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607024159_jfhm GCCTCTCTCTCTCTCTTGGCCTCTCCTGTCTG(+ATATAT)GATCCTTTATAGAACTGAGGTGGGGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607024157_d0kw TCCCAAAGAATGAAGCAATCTATGAAGAGAGG(+ATATAT)GTACCAGACAAAGATCCAAGACACCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607024156_g2as GGATTTGGGCTGGTGTCTTGGATCTTTGTCTG(+ATATAT)GTACCCTCTCTTCATAGATTGCTTCATTCTT NGG 10 8 17 8 24 0 100 5 10
    240607024155_kql0 CATTACTTTTTTCCTGTATATGAAGAAAATTG(+ATATAT)GTACCCTGTACTTACAATTTATTTATGTTAT NGG 10 8 17 8 24 0 100 5 10