Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024138_d1yt AAAGGATATGAAGTTGTGTGGGTAGGGGTGAG(+ATATAT)GATCCGGGAGAAGCTAGGAGAGGCGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607024136_sxco TTCCGTATGTTAGCTCAGTCTTGTATTCCTGG(+ATATAT)GATCCATCCCATTTGGTCATGGTGTCTAATC NGG 10 8 17 8 24 0 100 5 10
    240607024135_1f52 AAAATGATTAGACACCATGACCAAATGGGATG(+ATATAT)GATCCCAGGAATACAAGACTGAGCTAACATA NGG 10 8 17 8 24 0 100 5 10
    240607024133_m6nz ACCCGGCCTGGCTCTTGTGTTTTGACTAATTG(+ATATAT)GATCCCAGGTGCAAGCCTTGCTTGGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240607024131_51tv AGGGGTTGTGACCAAGCAAGGCTTGCACCTGG(+ATATAT)GATCCAATTAGTCAAAACACAAGAGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607024130_7iyf AACTACAGTTGTGGAATCAGACTGGCTGGCTG(+ATATAT)GATCCCAGCTCTGCCCCTTTGGCATCATCTT NGG 10 8 17 8 24 0 100 5 10
    240607024128_3ypj CACCTAAGATGATGCCAAAGGGGCAGAGCTGG(+ATATAT)GATCCAGCCAGCCAGTCTGATTCCACAACTG NGG 10 8 17 8 24 0 100 5 10
    240607024127_kh3j CTTTAACTGAGAAGCAGAGAGAGGGGCAAGTG(+ATATAT)GATCCTTGGTTTACTCAGGGAATCCAGACCA NGG 10 8 17 8 24 0 100 5 10
    240607024125_pbu5 CTGGCTGGTCTGGATTCCCTGAGTAAACCAAG(+ATATAT)GATCCACTTGCCCCTCTCTCTGCTTCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240607024122_15ax ATAACCCCATGCTCTGGCCCTGGCATCCCAAG(+ATATAT)GTACCACAAATGCCATCGGCCTTGCTGTGTG NGG 10 8 17 8 24 0 100 5 10