Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024035_1p47 TAAGATAAGCAATTTTCATGTCAGCCTTTCTG(+ATATAT)GTACCCAAATTGGATGTTCTTTATCCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607024033_2m5d GTGTATATAAGCAGTCCTCCCTGGGTACCCGG(+ATATAT)GATCCCTGTAGCAGCATCGAGGCGTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607024031_vvz5 TTATTGCAGGAACGCCTCGATGCTGCTACAGG(+ATATAT)GATCCCGGGTACCCAGGGAGGACTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240607024030_5abb CCATCCATGCTGGGTGCCATCCCACCTCAATG(+ATATAT)GTACCAATATAACCCCCAACAGACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240607024029_x97c GCCTTTATAAATGTCTGTTGGGGGTTATATTG(+ATATAT)GTACCATTGAGGTGGGATGGCACCCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607024026_fpnl CTCCACTCCTTCTCTACTCTTCTGGGCCCCAG(+ATATAT)GATCCTCTGCCTAGGGAATGGTGCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240607024025_telb CCAGCATGGATGGCACCATTCCCTAGGCAGAG(+ATATAT)GATCCTGGGGCCCAGAAGAGTAGAGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607024023_vl3k CAGGCCCAGTGTCACTCTTCTTGCTGCCTTTG(+ATATAT)GATCCAGATGTGAATTCTCTGCTACCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607024022_xa7f GTGCTGGAGAGGTAGCAGAGAATTCACATCTG(+ATATAT)GATCCAAAGGCAGCAAGAAGAGTGACACTGG NGG 10 8 17 8 24 0 100 5 10
    240607024020_khu0 ACTTTTGAGGCCACTGCCAGGACCCCAGCCTG(+ATATAT)GATCCGGGTTTTATCCAGGGACACCCAGCTA NGG 10 8 17 8 24 0 100 5 10