Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024018_86pw CTTCATAGCTGGGTGTCCCTGGATAAAACCCG(+ATATAT)GATCCAGGCTGGGGTCCTGGCAGTGGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240607024016_lawg CATTCCTGCCAGTTTGGCTAGCTCTAGATAGG(+ATATAT)GATCCTGCCTTGTATCCTGAGACCCAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607024015_9bv3 TCTGGTCCTCTGGGTCTCAGGATACAAGGCAG(+ATATAT)GATCCCTATCTAGAGCTAGCCAAACTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607024014_rzmv GAAGGAATCATACTAGAGAAGAAAAGTCAGAG(+ATATAT)GTACCACAATCAATAAGAAAACAGTAAATTA NGG 10 8 17 8 24 0 100 5 10
    240607024012_51e6 GTTTGTAATTTACTGTTTTCTTATTGATTGTG(+ATATAT)GTACCTCTGACTTTTCTTCTCTAGTATGATT NGG 10 8 17 8 24 0 100 5 10
    240607024011_5rqv GGGCGCTAACCCTCCCACCACTCGGCAGCGAG(+ATATAT)GATCCATGGTGGCTTTCATCATCTTTCATGG NGG 10 8 17 8 24 0 100 5 10
    240607024009_t6ig TGTCCCCATGAAAGATGATGAAAGCCACCATG(+ATATAT)GATCCTCGCTGCCGAGTGGTGGGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240607024008_kco2 GTTAGAATCACAACCAACTCTGTAATAAATGG(+ATATAT)GATCCTTTAACTCCTGACCTACTAAAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240607024007_tbu7 TCTTGGGGGCTTTAGTAGGTCAGGAGTTAAAG(+ATATAT)GATCCCATTTATTACAGAGTTGGTTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240607024005_dw8p ATGCGTGAGAACCCAATGTGAGAACAGGGAAG(+ATATAT)GATCCTTTCTGATTGTTGTGGCCTTGGGTTT NGG 10 8 17 8 24 0 100 5 10