Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023922_s3m6 TGGCTAAGGACTGTAATCTATTTCTGCATATG(+ATATAT)GATCCATCTTAGCTACCTTTATTATACTGGA NGG 10 8 17 8 24 0 100 5 10
    240607023920_irth TGGAGGAAACACTCTGTTGGAACCCTCCTGTG(+ATATAT)GATCCACACCTGACTTAGCACTTAAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240607023919_5ese GGAGGGTTAGCTTAAGTGCTAAGTCAGGTGTG(+ATATAT)GATCCACAGGAGGGTTCCAACAGAGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607023916_po6r TTGGGACCTGGCATCAGGACCTCTCCACCCAG(+ATATAT)GATCCTCCATGCCACATGGTCACTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607023915_ift0 CCTGAGGAGAGCAGTGACCATGTGGCATGGAG(+ATATAT)GATCCTGGGTGGAGAGGTCCTGATGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607023914_q84z GGCTGTGCTCTTAGGCTGTGCAAACATTGCAG(+ATATAT)GTACCCCTTTCCAGTTGCGAGCCACACATCA NGG 10 8 17 8 24 0 100 5 10
    240607023912_569y TAGGATGATGTGTGGCTCGCAACTGGAAAGGG(+ATATAT)GTACCTGCAATGTTTGCACAGCCTAAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607023911_m32h AGGGATGTCAGATAACTTGACCCAGGGCTATG(+ATATAT)GATCCACTGTGAAGGATGGCTTCCCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240607023910_hvmp CAGAAGACTCTGGGAAGCCATCCTTCACAGTG(+ATATAT)GATCCATAGCCCTGGGTCAAGTTATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240607023908_jagk TTTCTGGGCTCTGCTGGAAGGGCCCTGAGATG(+ATATAT)GTACCCGACCTGTGGAGACTGAGAGGGTACT NGG 10 8 17 8 24 0 100 5 10