Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023837_btzb CACTCCTGCCAATGGCCAGTGGAGAGCAGAAG(+ATATAT)GTACCCAGTCCACCTGCTTCTCTCAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240607023836_9602 AAGGACAGCGAGGACAGCCTTAATTCTCTCAG(+ATATAT)GTACCCAGCCTTCCATTTCCTTCCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607023834_5qee GAAAGGAAGGAAGGAAGGAAATGGAAGGCTGG(+ATATAT)GTACCTGAGAGAATTAAGGCTGTCCTCGCTG NGG 10 8 17 8 24 0 100 5 10
    240607023833_5lup ATATCTTGAAATACTGTCTCACTTATCTATAG(+ATATAT)GTACCAAATTCAGTCTTTAAGTGTCTACTCA NGG 10 8 17 8 24 0 100 5 10
    240607023832_17o6 AGTTATGAGTAGACACTTAAAGACTGAATTTG(+ATATAT)GTACCTATAGATAAGTGAGACAGTATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607023829_po3i TGCTTTATTTCTCGCAGCTGGATTGAACCCAG(+ATATAT)GATCCTGCATGCGCCAGTCAAGGACTTGACA NGG 10 8 17 8 24 0 100 5 10
    240607023828_cohf TTCATTGTCAAGTCCTTGACTGGCGCATGCAG(+ATATAT)GATCCTGGGTTCAATCCAGCTGCGAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607023827_f3rj ATTGTAAGAGACCCTTCCTGGGTACTAGCCTG(+ATATAT)GTACCTGGCTACTGAGCAGTGGGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607023825_8atj TTCTCTAGGCTTGCCCACTGCTCAGTAGCCAG(+ATATAT)GTACCAGGCTAGTACCCAGGAAGGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607023824_pbt6 AGGCTTGGGGAAGGCTGAGGCCCTGTTATTTG(+ATATAT)GATCCTGCCACCTCAGGGTCCCAGGCCCCTT NGG 10 8 17 8 24 0 100 5 10