Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023738_u862 GCCTGGCTTCTTAAGGCTGTGCACCACTATGG(+ATATAT)GATCCTTTGCAGAATGGTACTCATAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240607023737_6383 CAGTTACTAAGGCACATGACCGTGGTGAGGAG(+ATATAT)GATCCTCAGGACCTGCCGGAGCTGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240607023735_effc GGTGGCTGCAGGCAGCTCCGGCAGGTCCTGAG(+ATATAT)GATCCTCCTCACCACGGTCATGTGCCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240607023734_xh84 CTGTGTTTAGGTGTCACGTGTGAGATAATGTG(+ATATAT)GTACCAGGAAAGGAGCTATGTACTGAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607023732_n8hh GAAACAAGCCTCAGTACATAGCTCCTTTCCTG(+ATATAT)GTACCACATTATCTCACACGTGACACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607023730_ixo9 CAGCACAGTAGAGTCACCGAGGGGTATGGTAG(+ATATAT)GTACCAGAGGAACTATTCAGTCCTCATGGAA NGG 10 8 17 8 24 0 100 5 10
    240607023728_r4du GTCTGTTCCATGAGGACTGAATAGTTCCTCTG(+ATATAT)GTACCTACCATACCCCTCGGTGACTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240607023727_obnq CTCTCAATCGGGGCTGGAAAAGAACAAAGTGG(+ATATAT)GATCCCACACTTCTCCTGCACCCTCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607023725_o7id GCAACCTGCTGGAGGGTGCAGGAGAAGTGTGG(+ATATAT)GATCCCACTTTGTTCTTTTCCAGCCCCGATT NGG 10 8 17 8 24 0 100 5 10
    240607023724_g4gi GCCATCCATGGAATGAGTTTATGTGTACAAAG(+ATATAT)GATCCAGAAAAGGGGACATTTTAAAAAAATC NGG 10 8 17 8 24 0 100 5 10