Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023642_quez ACCACATGGTGGCTCACAACCATCTATAATGG(+ATATAT)GATCCAATGCCCCCTTCTGGAGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607023641_iisi GCTGTCTTCAGACACTCCAGAAGGGGGCATTG(+ATATAT)GATCCCATTATAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607023640_fzw2 GCTGTCTTCAGACACATCAGAAGAGGGTATCG(+ATATAT)GATCCCATTACAGATGTGGTTGCTGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240607023638_xpyw AATTCAACTCCCAGCAACCACATCTGTAATGG(+ATATAT)GATCCGATACCCTCTTCTGATGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607023637_7753 TAGGTAGCCTTGGCTGGCCTGGAACTCACAGG(+ATATAT)GATCCACCTGTCTCTGCCACCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607023635_6uud CAATTCCAGCACTTGGGTGGCAGAGACAGGTG(+ATATAT)GATCCCTGTGAGTTCCAGGCCAGCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607023634_0apu CAGGCAGACAGAGTAATGGAGAAGGAGCTGAG(+ATATAT)GATCCTACGTCTTGATCTGAAAGTGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607023633_srqd TTTCTCCTGACCACTTTCAGATCAAGACGTAG(+ATATAT)GATCCTCAGCTCCTTCTCCATTACTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607023632_itdl ATCAATGCTCTCCCAATAAACGTGTGCAGAAG(+ATATAT)GATCCTGTTGCAGCGTCGTTCTTCCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240607023630_knr4 TCAACTGGCCAGGAAGAACGACGCTGCAACAG(+ATATAT)GATCCTTCTGCACACGTTTATTGGGAGAGCA NGG 10 8 17 8 24 0 100 5 10