Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023600_jpjg AATCTTATGTGTAGACAAGGACACACACACAG(+ATATAT)GTACCTATGCATTCATATACAGTAGACCAAT NGG 10 8 17 8 24 0 100 5 10
    240607023559_31lx TTCCCATTGGTCTACTGTATATGAATGCATAG(+ATATAT)GTACCTGTGTGTGTGTCCTTGTCTACACATA NGG 10 8 17 8 24 0 100 5 10
    240607023557_cxlr CTGAAAACATAACTTAAATAGACTGACTGGAG(+ATATAT)GATCCGCCACACACGTGTTCATGGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607023556_4hrs CACTGTTCCCATCCATGAACACGTGTGTGGCG(+ATATAT)GATCCTCCAGTCAGTCTATTTAAGTTATGTT NGG 10 8 17 8 24 0 100 5 10
    240607023554_vjd0 CTGGATTGGAACTCATTAAGACCAACAGCCAG(+ATATAT)GATCCCGATTTTGAGCCTAAACTTTTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607023553_qvuo AGGAAGGAACAAAAGTTTAGGCTCAAAATCGG(+ATATAT)GATCCTGGCTGTTGGTCTTAATGAGTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240607023551_7xeb TATAATTTATGCAGAGAAATTTCACTTACATG(+ATATAT)GTACCTTTTCCAGTCTCAACATCTCATCATA NGG 10 8 17 8 24 0 100 5 10
    240607023550_vbnq CATTTTATGATGAGATGTTGAGACTGGAAAAG(+ATATAT)GTACCATGTAAGTGAAATTTCTCTGCATAAA NGG 10 8 17 8 24 0 100 5 10
    240607023549_mdk0 GGAAGGTTGGTCCCACAAGATGCACTGAAATG(+ATATAT)GATCCCCACCCAATAAAGATGGTATTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607023547_ol0f CATATGTAACAATACCATCTTTATTGGGTGGG(+ATATAT)GATCCATTTCAGTGCATCTTGTGGGACCAAC NGG 10 8 17 8 24 0 100 5 10