Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023532_f3tq ATTTGAAATAAAGGTTTTAATTCTGAAGTAGG(+ATATAT)GATCCTTAAATAAGGTCTAATCCAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240607023531_eq4v GGAGATATAAATTGGATTAGACCTTATTTAAG(+ATATAT)GATCCCTACTTCAGAATTAAAACCTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240607023530_2lnz AGGGAACCCAGGGTCACCTGCAGGTGACAGAG(+ATATAT)GTACCCGCCTCCACTTAATGTCCATCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240607023528_wogj ACCCCAGCTTGATGGACATTAAGTGGAGGCGG(+ATATAT)GTACCTCTGTCACCTGCAGGTGACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607023526_ses8 GGTGGGCTAAGTTGCCAAAAACGACAATCAGG(+ATATAT)GATCCAATAAGCAATCTTGGTCACTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607023525_qmw3 AAATTTACTTAAGTGACCAAGATTGCTTATTG(+ATATAT)GATCCCTGATTGTCGTTTTTGGCAACTTAGC NGG 10 8 17 8 24 0 100 5 10
    240607023524_ufle TTCTTAGTATTCAGAATTGATGTGTTTTATTG(+ATATAT)GTACCAGCTGTCTTAATGCATTTATTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607023522_8sl4 AACTGTTCTTAATAAATGCATTAAGACAGCTG(+ATATAT)GTACCAATAAAACACATCAATTCTGAATACT NGG 10 8 17 8 24 0 100 5 10
    240607023521_965p GTCTCACACCTCAGTCCCTTGGTCACACTAAG(+ATATAT)GTACCCAAGATGTTGGCCAGGATTACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240607023520_x35j GGAATCTCTAGTAATCCTGGCCAACATCTTGG(+ATATAT)GTACCTTAGTGTGACCAAGGGACTGAGGTGT NGG 10 8 17 8 24 0 100 5 10