Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023546_d2ja TACATAAACCAATGCTTGAAGATGCTGCAAAG(+ATATAT)GATCCTCTAGTGATTCCTGATGAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240607023545_22vl CTATTCTGATTCCTCATCAGGAATCACTAGAG(+ATATAT)GATCCTTTGCAGCATCTTCAAGCATTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607023544_jyt3 TCCTGTATCTTGCCTTTGTACAAGAGCATATG(+ATATAT)GTACCTTTTTGAATAAAATAACATTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240607023543_w2u2 TAAATATGTTAAATGTTATTTTATTCAAAAAG(+ATATAT)GTACCATATGCTCTTGTACAAAGGCAAGATA NGG 10 8 17 8 24 0 100 5 10
    240607023541_z9ha AGGAAGCTCTGGCAATTCTGGTTCTTAACCAG(+ATATAT)GATCCAGGAGGCATCACTGGGAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240607023539_05b8 TTGAAATCACACTTTCCCAGTGATGCCTCCTG(+ATATAT)GATCCTGGTTAAGAACCAGAATTGCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607023538_j482 GATCGTTGAATCTCTCTCATCCCAGTGCAATG(+ATATAT)GATCCACAAATCCGTGGGATGCAAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240607023537_f4dt AGTTCTCTGCTTTTGCATCCCACGGATTTGTG(+ATATAT)GATCCATTGCACTGGGATGAGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240607023536_fybm ACAAATAGCTCCCCAGGATCTGATTAAGATAG(+ATATAT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607023534_0pap AAAATGTCCTTGATAATAAATGGTATGGCCCG(+ATATAT)GATCCTATCTTAATCAGATCCTGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10