Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023518_5k5l AGTTAGCTGGGGCAGAGGTATGGTTGGAGTAG(+ATATAT)GTACCTAGGACGGCAACCCCTTCGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240607023516_1ctu ACCCCCCTATTACGAAGGGGTTGCCGTCCTAG(+ATATAT)GTACCTACTCCAACCATACCTCTGCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607023515_5vsp TGACAGTAACTGATCTGCATTAGTTAGGAGTG(+ATATAT)GTACCGCTTTCATCAGTTCAATGATGCTACT NGG 10 8 17 8 24 0 100 5 10
    240607023514_y4k7 TGTAAAGTAGCATCATTGAACTGATGAAAGCG(+ATATAT)GTACCACTCCTAACTAATGCAGATCAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607023513_18u8 GAAGAACTCTTATGAATACATTAAAGTAAAAG(+ATATAT)GATCCCTAAAACGATTAAGCATACAGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240607023511_sv8b ATTGTCCAACCTGTATGCTTAATCGTTTTAGG(+ATATAT)GATCCTTTTACTTTAATGTATTCATAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607023510_4pf4 TTCTGTGCCTCAAGGAAAAGGAGCCCTCTTAG(+ATATAT)GTACCGCCACAACATGGATGATCCAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240607023509_zmwq AGGCAACAGTCTGGATCATCCATGTTGTGGCG(+ATATAT)GTACCTAAGAGGGCTCCTTTTCCTTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607023507_zoff TTGGATCCAGTGACATAGCTAATAGGGTGAAG(+ATATAT)GTACCTGCCACCAACTCTGATAATTTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240607023506_eckx GATTGAACTCAAATTATCAGAGTTGGTGGCAG(+ATATAT)GTACCTTCACCCTATTAGCTATGTCACTGGA NGG 10 8 17 8 24 0 100 5 10