Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023420_0gz3 AATGAGCTACCAAGCTATAAAAACTCACAGAG(+ATATAT)GATCCTTAAAGAGTGTTAGTGAATAATTCCA NGG 10 8 17 8 24 0 100 5 10
    240607023418_5ytf CTAGTTGGAATTATTCACTAACACTCTTTAAG(+ATATAT)GATCCTCTGTGAGTTTTTATAGCTTGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240607023417_62q6 GTGCAGCATGTCTCATGCATCGTATTGAACTG(+ATATAT)GATCCCGCACAGGGAATAATGTAATCTATTG NGG 10 8 17 8 24 0 100 5 10
    240607023416_n1ni ATCATCAATAGATTACATTATTCCCTGTGCGG(+ATATAT)GATCCAGTTCAATACGATGCATGAGACATGC NGG 10 8 17 8 24 0 100 5 10
    240607023414_01uf GCATGAGAGTGTGCAGTGGTGCCTGAGTCCTG(+ATATAT)GTACCTTGAAAAGCTTTGTCTAAGAATGCAA NGG 10 8 17 8 24 0 100 5 10
    240607023413_10td TGTTGTTGCATTCTTAGACAAAGCTTTTCAAG(+ATATAT)GTACCAGGACTCAGGCACCACTGCACACTCT NGG 10 8 17 8 24 0 100 5 10
    240607023411_cljx GCAGCTGGTTCTCTCAGCATGTGGGTCTCCAG(+ATATAT)GTACCAAAAGGCATGAGGCTTGGCAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240607023409_1jfb AAACCGCTTGCTGCCAAGCCTCATGCCTTTTG(+ATATAT)GTACCTGGAGACCCACATGCTGAGAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240607023408_1y9h TTACTTGATACACCGTGGAATGAAAATGTAAG(+ATATAT)GTACCTTTCAGTATGTGCTCTGAGCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240607023407_09u9 AAAAAATTAAAGCTCAGAGCACATACTGAAAG(+ATATAT)GTACCTTACATTTTCATTCCACGGTGTATCA NGG 10 8 17 8 24 0 100 5 10