Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023334_qwzn TTTTCTGCCAAATAACGTTGACCAGTTATATG(+ATATAT)GTACCAAGAAATGCCCCTGCCCCTCCCTATA NGG 10 8 17 8 24 0 100 5 10
    240607023333_ucx6 TGATCTATAGGGAGGGGCAGGGGCATTTCTTG(+ATATAT)GTACCATATAACTGGTCAACGTTATTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607023331_divz TCATATGTGCAGCTGCCCTGCCATGTCCAGAG(+ATATAT)GATCCTGCTTCCTCGTATTCGTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240607023330_zrqx GACAGAGGTGGTGAACGAATACGAGGAAGCAG(+ATATAT)GATCCTCTGGACATGGCAGGGCAGCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240607023329_bqbu TGGAATTATGAAGGTACTGGTTTATGGGTGAG(+ATATAT)GATCCTTGGCATTATATTTTGGCTGGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607023327_ozn6 AAGAAAGAGGCCAGCCAAAATATAATGCCAAG(+ATATAT)GATCCTCACCCATAAACCAGTACCTTCATAA NGG 10 8 17 8 24 0 100 5 10
    240607023325_wn74 CTGGGCAGGAGCAGCCAGCCCTCAGGAGGCTG(+ATATAT)GTACCAAAGGCATGAATGTTCTCAGGGGATA NGG 10 8 17 8 24 0 100 5 10
    240607023324_h9y6 CATCTTATCCCCTGAGAACATTCATGCCTTTG(+ATATAT)GTACCAGCCTCCTGAGGGCTGGCTGCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607023322_u8n2 TGAATTCCATGTGTGTTTTATGACCTAAATGG(+ATATAT)GATCCTGACACCTGCTGTGTGGCCACCACGA NGG 10 8 17 8 24 0 100 5 10
    240607023321_nlxw AAGAATCGTGGTGGCCACACAGCAGGTGTCAG(+ATATAT)GATCCCATTTAGGTCATAAAACACACATGGA NGG 10 8 17 8 24 0 100 5 10