Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023257_bgtr CTTGTAAAAGCCAAGGACAGGATCTCCCTAAG(+ATATAT)GATCCAAGTACCCATTGTTTGAAGTATACTT NGG 10 8 17 8 24 0 100 5 10
    240607023256_r9f4 GAAGAAAGTATACTTCAAACAATGGGTACTTG(+ATATAT)GATCCTTAGGGAGATCCTGTCCTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240607023254_p003 TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+ATATAT)GTACCTGCACTTGACAGGGCCTGGCACATGG NGG 10 8 17 8 24 0 100 5 10
    240607023253_18d5 ACTCCCCATGTGCCAGGCCCTGTCAAGTGCAG(+ATATAT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607023252_5p1p TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+ATATAT)GTACCTGCACTTGACAGGGCCTGGTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240607023251_hsrg ACTCCCCATGCACCAGGCCCTGTCAAGTGCAG(+ATATAT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607023249_yhn7 ACAGCCTGTCTCTGCTCCCCATGAATCCTAGG(+ATATAT)GATCCCCATGCAGGTTCACAAGCTTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607023247_8svu GCTTGCTACACAAGCTTGTGAACCTGCATGGG(+ATATAT)GATCCCTAGGATTCATGGGGAGCAGAGACAG NGG 10 8 17 8 24 0 100 5 10
    240607023246_ttzt TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+ATATAT)GTACCTGCACTTGACAGGGCCTTAAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240607023245_lgu6 ACTCCCCATGCTTAAGGCCCTGTCAAGTGCAG(+ATATAT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10