Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023159_fpmr GACTCCAGGACATGAAATGCTGTTCTCACTTG(+ATATAT)GTACCACGTTTTCTACACAGAACCACATCCT NGG 10 8 17 8 24 0 100 5 10
    240607023158_1yl7 TGGTAAGGATGTGGTTCTGTGTAGAAAACGTG(+ATATAT)GTACCAAGTGAGAACAGCATTTCATGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607023157_u1y5 TATGCAGAATCCTTGGGAAGCTAGAATATGGG(+ATATAT)GTACCATCCTCATAGCCCCAGAGAGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607023155_33pc GGCTTCTCCCACTCTCTGGGGCTATGAGGATG(+ATATAT)GTACCCCATATTCTAGCTTCCCAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240607023154_v3d6 AGTGAGCCATCTGTGAGATCTCCATAGTCTAG(+ATATAT)GATCCACTGGTCCCAGGGGGACTGAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240607023152_5srh CTATCAGTCACTCAGTCCCCCTGGGACCAGTG(+ATATAT)GATCCTAGACTATGGAGATCTCACAGATGGC NGG 10 8 17 8 24 0 100 5 10
    240607023151_2249 GAGGGTCCAGCCACCACTGTCTTCTTTCTCTG(+ATATAT)GATCCAGAACAAGGAAATCTAGATGAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240607023150_3tfh GCCACCCGGCTCATCTAGATTTCCTTGTTCTG(+ATATAT)GATCCAGAGAAAGAAGACAGTGGTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607023149_xy2o GTGTGCAGTTCAATCCCGAGGACTGGAATTTG(+ATATAT)GATCCCACCACACACATAACAAGCCACATAT NGG 10 8 17 8 24 0 100 5 10
    240607023147_98eq ACAGAATATGTGGCTTGTTATGTGTGTGGTGG(+ATATAT)GATCCAAATTCCAGTCCTCGGGATTGAACTG NGG 10 8 17 8 24 0 100 5 10