Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023146_ol0q GCCCCAAGGTCACGTGTTAGGATACTATATGG(+ATATAT)GTACCATACATTTCAAAGTTGCTCATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607023144_ce2k GCTCATTTCCATGAGCAACTTTGAAATGTATG(+ATATAT)GTACCCATATAGTATCCTAACACGTGACCTT NGG 10 8 17 8 24 0 100 5 10
    240607023143_da6d TTATACCAGTGCCCGGGAGATGAGGATGGGTG(+ATATAT)GATCCGTGGTGCTGGCTAGACAGACAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607023141_llt5 TCTACTGGGCTGTCTGTCTAGCCAGCACCACG(+ATATAT)GATCCACCCATCCTCATCTCCCGGGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240607023140_khw6 AACTGAGCTCAAGGCCCAGGGACACATAGCAG(+ATATAT)GTACCAGGAGCAAGAGAGAGCCCCAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607023138_2upa GAACTCCTTTCTGGGGCTCTCTCTTGCTCCTG(+ATATAT)GTACCTGCTATGTGTCCCTGGGCCTTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607023137_l4wx TGTGTATGTGTAGGTGAGAGAACGATTTTCAG(+ATATAT)GATCCTATCCACTCTTCCCTCCATGTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607023136_zjjx ACCTGGTCCTACATGGAGGGAAGAGTGGATAG(+ATATAT)GATCCTGAAAATCGTTCTCTCACCTACACAT NGG 10 8 17 8 24 0 100 5 10
    240607023135_rhpx TCATTCTCACACAACCCCCTATCTATGGAATG(+ATATAT)GTACCATCCACAGTGGGCTGGGTCTTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240607023134_mdh6 TTTGCCTAGAAAGACCCAGCCCACTGTGGATG(+ATATAT)GTACCATTCCATAGATAGGGGGTTGTGTGAG NGG 10 8 17 8 24 0 100 5 10