Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023133_9byg CCAACTGAGCAGGTAATGTGAGTATGTGAATG(+ATATAT)GATCCTAAGTTGGGTAAACCAATTCTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240607023131_aqi8 TTAATTTTTGAGAATTGGTTTACCCAACTTAG(+ATATAT)GATCCATTCACATACTCACATTACCTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607023130_zo96 GGATGAGAAGTCTGAACTCTACTGCACAACTG(+ATATAT)GATCCCTAGATTGAGCAGGCTTTATCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240607023129_ozrb GCTTTTGAAAGATAAAGCCTGCTCAATCTAGG(+ATATAT)GATCCAGTTGTGCAGTAGAGTTCAGACTTCT NGG 10 8 17 8 24 0 100 5 10
    240607023127_vqu0 CTGATGCCCATAGAGATCAGAAGAGGGCACTG(+ATATAT)GATCCCATGCATCGGGAGTATTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607023126_em9d GCCCACAACTATCCAATACTCCCGATGCATGG(+ATATAT)GATCCAGTGCCCTCTTCTGATCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240607023125_kngu TCAACTTCCGGGAGGAAGGATGACTAGAGATG(+ATATAT)GTACCAAAGGTGGGAGACGCTTGGGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240607023123_7d73 TGGCAAGGGTGCCCAAGCGTCTCCCACCTTTG(+ATATAT)GTACCATCTCTAGTCATCCTTCCTCCCGGAA NGG 10 8 17 8 24 0 100 5 10
    240607023121_8quw AAATAAATCAGAGGAACCCTGGACCCAGCCAG(+ATATAT)GATCCTACCCTTTGGGGGATTGGAGGCGTGC NGG 10 8 17 8 24 0 100 5 10
    240607023120_wix6 AGGCTGCACGCCTCCAATCCCCCAAAGGGTAG(+ATATAT)GATCCTGGCTGGGTCCAGGGTTCCTCTGATT NGG 10 8 17 8 24 0 100 5 10