Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023034_uf1y GCAACTATTTCTAGGGCAGAGCAAAGGCCTGG(+ATATAT)GATCCGGGATGAGCTTCAGGTTCTCCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607023031_5pcl CACAGGAGCTGGAGAACCTGAAGCTCATCCCG(+ATATAT)GATCCCAGGCCTTTGCTCTGCCCTAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607023029_fs41 GTGTCAATCACAGGGTTCTGACAGAAACCTGG(+ATATAT)GATCCCTTTGAAGGGCAGGCAGCCGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607023028_xvtq GCGGAGCCTTGCGGCTGCCTGCCCTTCAAAGG(+ATATAT)GATCCCAGGTTTCTGTCAGAACCCTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240607023026_ilfo TGGAGGCATATCAGAACCACAGTTAGGGAAGG(+ATATAT)GATCCAAGTTGGTTGGAGAACAGCTAGGACT NGG 10 8 17 8 24 0 100 5 10
    240607023025_bjnt CACCCAGTCCTAGCTGTTCTCCAACCAACTTG(+ATATAT)GATCCCTTCCCTAACTGTGGTTCTGATATGC NGG 10 8 17 8 24 0 100 5 10
    240607023023_w1g8 AGGGCTTTGTCCCCTTTCTGTAAGTCTCTTGG(+ATATAT)GATCCTGTGTAGGAGCTGTTCTCATTAAACA NGG 10 8 17 8 24 0 100 5 10
    240607023022_ltl1 GTTGATGTTTAATGAGAACAGCTCCTACACAG(+ATATAT)GATCCCAAGAGACTTACAGAAAGGGGACAAA NGG 10 8 17 8 24 0 100 5 10
    240607023020_ab73 GGCCATAGCTGAGCAGAGAAGGTTCAATGCAG(+ATATAT)GTACCCATCTGATGGGTTAAGATGGATGTAA NGG 10 8 17 8 24 0 100 5 10
    240607023019_81zw TAGTATTACATCCATCTTAACCCATCAGATGG(+ATATAT)GTACCTGCATTGAACCTTCTCTGCTCAGCTA NGG 10 8 17 8 24 0 100 5 10