Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022945_o7yl TTGGGAGCAGTTGTGGAACAGAGGACAGATCG(+ATATAT)GTACCATACAGAGGCATGGGTTCGCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240607022944_jrdo GAGCAGCTACAGCGAACCCATGCCTCTGTATG(+ATATAT)GTACCGATCTGTCCTCTGTTCCACAACTGCT NGG 10 8 17 8 24 0 100 5 10
    240607022942_c2hi GCTCTCTGAGGGCAACCAGGCCTTGTGTCCTG(+ATATAT)GTACCTGTGACACTGTTGTGGTCACAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240607022941_dyj1 AGGGCACTGCTGTGACCACAACAGTGTCACAG(+ATATAT)GTACCAGGACACAAGGCCTGGTTGCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607022939_ifus AAGCATCAGTTGTGTTCAGAGCTGTGGTCCTG(+ATATAT)GTACCCCTAGTTGACATCCTGTTTCTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240607022937_g4xg GTACGTAAGCAGAAACAGGATGTCAACTAGGG(+ATATAT)GTACCAGGACCACAGCTCTGAACACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240607022935_il22 GGGAGTGAATCGGGGTGAGCAGGCTTTGCCCG(+ATATAT)GATCCCAGAACTCTAGTCCGTAAGTCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240607022933_mnpa TGGTTATGAGGACTTACGGACTAGAGTTCTGG(+ATATAT)GATCCGGGCAAAGCCTGCTCACCCCGATTCA NGG 10 8 17 8 24 0 100 5 10
    240607022932_g52o CTTGCTGTGCAGGAGCCACATGGTTCTTCTGG(+ATATAT)GTACCAGGGCCAGGCCTGGACACCACACAGT NGG 10 8 17 8 24 0 100 5 10
    240607022930_2oft AACTAACTGTGTGGTGTCCAGGCCTGGCCCTG(+ATATAT)GTACCCAGAAGAACCATGTGGCTCCTGCACA NGG 10 8 17 8 24 0 100 5 10