Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023002_ge22 ACTGCATTGGTAGGGTACAAAGGTGTTCAGAG(+ATATAT)GATCCCAGTCTGAGTGATTAGCCTAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240607023000_zkkb TGCTTCAGTCTTAGGCTAATCACTCAGACTGG(+ATATAT)GATCCTCTGAACACCTTTGTACCCTACCAAT NGG 10 8 17 8 24 0 100 5 10
    240607022959_5cn1 CTCCCACGACCTCGGTCCCAGTTGGACACCAG(+ATATAT)GTACCTCTGGAAAATGAGACAACCGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607022957_mauy CACGCACAGCCCGGTTGTCTCATTTTCCAGAG(+ATATAT)GTACCTGGTGTCCAACTGGGACCGAGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240607022955_ruqf GGTGGAAGGTAGTCGGATCTTCCAGAAGGCTG(+ATATAT)GATCCTCACGAGGTTGATCAACCTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607022954_fksk CTATCTTTAGACAGGTTGATCAACCTCGTGAG(+ATATAT)GATCCAGCCTTCTGGAAGATCCGACTACCTT NGG 10 8 17 8 24 0 100 5 10
    240607022953_h8te GCTGGGATCATGGATAAGTGTTAACATGCCAG(+ATATAT)GATCCTCTTCTATCTTTAAGTCTAAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240607022951_5pif AAGTCACTATCTTAGACTTAAAGATAGAAGAG(+ATATAT)GATCCTGGCATGTTAACACTTATCCATGATC NGG 10 8 17 8 24 0 100 5 10
    240607022949_tlif TGCTGGACCTGGGCAGGATGTACTCCCCTGGG(+ATATAT)GTACCGGGGCCTGCCTGCGGCTTACCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240607022946_6f9r TGCAGCAGAAGGTAAGCCGCAGGCAGGCCCCG(+ATATAT)GTACCCCAGGGGAGTACATCCTGCCCAGGTC NGG 10 8 17 8 24 0 100 5 10