Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022852_rqcr ACGTACATTGAGCTCCAGAAGGAAAAGAGAGG(+ATATAT)GATCCTAACCAAGGGCCACCAGGAAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607022851_a7ri TTGCTTGCTGCTTCCTGGTGGCCCTTGGTTAG(+ATATAT)GATCCCTCTCTTTTCCTTCTGGAGCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240607022850_o5xy CAGGAGTCCCTGAGGTTCCCAGCTGCTGTCAG(+ATATAT)GATCCTGTGAGTTGGGGCAAGCAGGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607022848_9j1z GGTGGCAGCATCCTGCTTGCCCCAACTCACAG(+ATATAT)GATCCTGACAGCAGCTGGGAACCTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240607022847_127x CTGTCACATGCAGCAGACAGGCAGCATTAAGG(+ATATAT)GTACCCTAGCTCTGTTAGGGCAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607022845_973y ACATTCCCAGGCTCTGCCCTAACAGAGCTAGG(+ATATAT)GTACCCTTAATGCTGCCTGTCTGCTGCATGT NGG 10 8 17 8 24 0 100 5 10
    240607022844_y3bf GGCGGCACTCGCCCCTCCTGGGGATACAGCAG(+ATATAT)GATCCGGCCTCAAGAGGCTTCAGGTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240607022842_a44p GAGTGTCAAGCACCTGAAGCCTCTTGAGGCCG(+ATATAT)GATCCTGCTGTATCCCCAGGAGGGGCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240607022840_cwed GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+ATATAT)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607022839_3zhg GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+ATATAT)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10