Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022928_zd2e CATACTCCTGGGCTGTAGAGTGGGGCAGGCCG(+ATATAT)GTACCGGCCCGCAGGCTGATCCTGGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607022926_gbvz CAGGATGAGAACCAGGATCAGCCTGCGGGCCG(+ATATAT)GTACCGGCCTGCCCCACTCTACAGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607022923_q8ui CCCACACCCTGGTGGCTAGAGGAGAGTAGGGG(+ATATAT)GTACCCTACAGCCACTGGGGGCTCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607022922_l8fe GCCTGCAGGCAGGAGCCCCCAGTGGCTGTAGG(+ATATAT)GTACCCCCTACTCTCCTCTAGCCACCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240607022920_fujd CTTGAGGCAGCCACCTCACCAGGAACACCTGG(+ATATAT)GATCCAGCCCAGGCTGGGCCGATGTGAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607022918_ild1 AGCCTCGCCTCACATCGGCCCAGCCTGGGCTG(+ATATAT)GATCCCAGGTGTTCCTGGTGAGGTGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607022915_zey9 CAGCTGCCTAAAGCTGGGCCTGGCTGGCCGGG(+ATATAT)GATCCACTTAGTCCAGAAGGAGAAAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240607022914_3dif ACCTTTGTCTCTTTCTCCTTCTGGACTAAGTG(+ATATAT)GATCCCCGGCCAGCCAGGCCCAGCTTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607022913_m0il GTTCTGCTTGCCCAGGACAGCTCAGCCTGTAG(+ATATAT)GATCCTCTCACAGGGAAGATGCTCTTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240607022912_yfd4 TGTTTTAGCCAAGAGCATCTTCCCTGTGAGAG(+ATATAT)GATCCTACAGGCTGAGCTGTCCTGGGCAAGC NGG 10 8 17 8 24 0 100 5 10