Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023017_7tto CACACCAGTTGTCACGATACCTGGAGAGGTGG(+ATATAT)GATCCTCCTAAGTACCTCAGTGGAAACCCTT NGG 10 8 17 8 24 0 100 5 10
    240607023015_k0qb AAAGGAAGGGTTTCCACTGAGGTACTTAGGAG(+ATATAT)GATCCCACCTCTCCAGGTATCGTGACAACTG NGG 10 8 17 8 24 0 100 5 10
    240607023014_fx2n CATCTCCAGATACACAGTATGTCCAGTTGGTG(+ATATAT)GTACCTGAGCCCACCCTTGACTAGGGCAGCG NGG 10 8 17 8 24 0 100 5 10
    240607023012_6bu7 CAGGCCGCTGCCCTAGTCAAGGGTGGGCTCAG(+ATATAT)GTACCACCAACTGGACATACTGTGTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240607023011_bnn1 CCATGTGGGCTCCATGGATCAAACTAATGGTG(+ATATAT)GTACCAATAAGCCATCTTGCTGGCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240607023010_97d5 TTGAATTCTAGGGCCAGCAAGATGGCTTATTG(+ATATAT)GTACCACCATTAGTTTGATCCATGGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607023009_z025 GCCTGGGCCATGGCCTCTTCTAAGAAAAGTTG(+ATATAT)GTACCAACCACTAATGGTCCTTTTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607023007_fw6m CTAGAAGAGCAGAAAAGGACCATTAGTGGTTG(+ATATAT)GTACCAACTTTTCTTAGAAGAGGCCATGGCC NGG 10 8 17 8 24 0 100 5 10
    240607023005_97hu TCCTGGCATCCTGCAGCCTTTAAGACCTGGGG(+ATATAT)GATCCCTTACAGAAACAGTCTTAGCCTTTCG NGG 10 8 17 8 24 0 100 5 10
    240607023003_msv1 AGGCTCGAAAGGCTAAGACTGTTTCTGTAAGG(+ATATAT)GATCCCCCAGGTCTTAAAGGCTGCAGGATGC NGG 10 8 17 8 24 0 100 5 10