Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022806_m3rs TGAAAGGCTTTCCCCAAAATGCCAGTGCCCAG(+ATATAT)GATCCTGAGGGGAGGTCGGGGGTGGAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240607022804_1mcq AGCCGGACTCTCCACCCCCGACCTCCCCTCAG(+ATATAT)GATCCTGGGCACTGGCATTTTGGGGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607022803_9ahl CAGCCATCTGCTGAGTGTTAGTTGAGAAGATG(+ATATAT)GATCCTAAGCCAGCTGAAGAATGAGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607022802_1p5z CACAGCCTGCACTCATTCTTCAGCTGGCTTAG(+ATATAT)GATCCATCTTCTCAACTAACACTCAGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240607022800_f1b1 CCAAACGTGGGCTGCTGCAATCCCTCAGCCTG(+ATATAT)GATCCTGAGCTACAGAAAAGAAGGCGGTCAA NGG 10 8 17 8 24 0 100 5 10
    240607022759_22fj GCCAGTTGACCGCCTTCTTTTCTGTAGCTCAG(+ATATAT)GATCCAGGCTGAGGGATTGCAGCAGCCCACG NGG 10 8 17 8 24 0 100 5 10
    240607022757_qpwo CATGGGAGAGGCCAGTCCACCTGACATGCCAG(+ATATAT)GATCCAAGAAGCAGTACTTTTAATTGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607022756_2hws GGGTTTCACCCAATTAAAAGTACTGCTTCTTG(+ATATAT)GATCCTGGCATGTCAGGTGGACTGGCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240607022755_ifci TCAGCTGGTGCAGGGTGAGAGAGCCAGAAATG(+ATATAT)GTACCACACCTGCCTTCCCACCTGGCTAACT NGG 10 8 17 8 24 0 100 5 10
    240607022754_tvat TGGCCAGTTAGCCAGGTGGGAAGGCAGGTGTG(+ATATAT)GTACCATTTCTGGCTCTCTCACCCTGCACCA NGG 10 8 17 8 24 0 100 5 10