Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022707_ohnv CCTAACCTTCCTTACCACACCAGCCTGGGACG(+ATATAT)GATCCGAGCCCCAGCGGGAAGCCATTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607022706_eb90 CCTGGAAGAGAATGGCTTCCCGCTGGGGCTCG(+ATATAT)GATCCGTCCCAGGCTGGTGTGGTAAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607022704_yzzq CCAGCCCTTGACACAGTCCAGCTGTGCCACAG(+ATATAT)GTACCACACTGGGAACTTGTCACCCTCAAGA NGG 10 8 17 8 24 0 100 5 10
    240607022703_9qjo AAGTGTCTTGAGGGTGACAAGTTCCCAGTGTG(+ATATAT)GTACCTGTGGCACAGCTGGACTGTGTCAAGG NGG 10 8 17 8 24 0 100 5 10
    240607022701_pa71 TTTAGCTCATAGACTAGCAGAGCCACCCTTAG(+ATATAT)GATCCTTCCTCTCCCATGTTTACCCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607022659_9yyz CGGAGCATGCTGGGTAAACATGGGAGAGGAAG(+ATATAT)GATCCTAAGGGTGGCTCTGCTAGTCTATGAG NGG 10 8 17 8 24 0 100 5 10
    240607022658_8akr AGCTTGCCTCCTGATACCAAATTATAATTTTG(+ATATAT)GATCCTTTAAGGATCTTACATTTAATTAATC NGG 10 8 17 8 24 0 100 5 10
    240607022657_yx1e TAAATGATTAATTAAATGTAAGATCCTTAAAG(+ATATAT)GATCCAAAATTATAATTTGGTATCAGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240607022656_icyx AAGCTGAGCAAGGCAGGGGAAGCGAGCTTCTG(+ATATAT)GATCCTGATGTTTTGTGCAGGAATAGAAATG NGG 10 8 17 8 24 0 100 5 10
    240607022654_p3n8 CTTAGCATTTCTATTCCTGCACAAAACATCAG(+ATATAT)GATCCAGAAGCTCGCTTCCCCTGCCTTGCTC NGG 10 8 17 8 24 0 100 5 10