Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022653_lsbm AGCAGGAGGCTCAATCCAGAAGAAACCTTGAG(+ATATAT)GATCCACCAAATAGGTATGAGCCCACAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607022652_tpab CTGACATTCTGTGGGCTCATACCTATTTGGTG(+ATATAT)GATCCTCAAGGTTTCTTCTGGATTGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607022650_qfok GGAGGCAGGAACGCTTTGAAAAAGGCAAACGG(+ATATAT)GATCCACTGAACCTCATCTTTATCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607022649_zz7e CCTCCACCCAAGGATAAAGATGAGGTTCAGTG(+ATATAT)GATCCCGTTTGCCTTTTTCAAAGCGTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607022647_8g49 CACGGGGTGGGGGTGAGGGAAGCAGGCAGGTG(+ATATAT)GATCCCTGAGGCTCACTGGACAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607022646_mg15 TAGGTCAGGCTGGCTGTCCAGTGAGCCTCAGG(+ATATAT)GATCCACCTGCCTGCTTCCCTCACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607022644_hmzd CAGGAGGATCTCAAATTCAAGACCACTCTGGG(+ATATAT)GTACCCAAGGAGACCCTGCCTCAAAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240607022642_g8z4 ATATTTGTGCTTTTGAGGCAGGGTCTCCTTGG(+ATATAT)GTACCCCAGAGTGGTCTTGAATTTGAGATCC NGG 10 8 17 8 24 0 100 5 10
    240607022640_sa3j TTGAATAAATAGTTCAGGCTTTTCTCATCTCG(+ATATAT)GTACCCAGGCTCCTGGAGACTGAAGATCAAC NGG 10 8 17 8 24 0 100 5 10
    240607022638_ep4w GGGTAGTTGATCTTCAGTCTCCAGGAGCCTGG(+ATATAT)GTACCGAGATGAGAAAAGCCTGAACTATTTA NGG 10 8 17 8 24 0 100 5 10