Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022554_ei7p TGTTTGCCTTGTTCTCCAGGGGGGCTGCTGGG(+ATATAT)GTACCCTGGCTTGGGGTGCCCTGTCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607022552_7gg1 GCTTCAACCAAGACAGGGCACCCCAAGCCAGG(+ATATAT)GTACCCCAGCAGCCCCCCTGGAGAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607022550_kfli AGTGCAGTCCAGGCTAGCCTTGATCTTAACGG(+ATATAT)GATCCCTGTGCTGACTCAGCCTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607022548_b9cf AAAAAAAAAAAAAAAGGCTGAGTCAGCACAGG(+ATATAT)GATCCCGTTAAGATCAAGGCTAGCCTGGACT NGG 10 8 17 8 24 0 100 5 10
    240607022547_w701 GCCAGGGACTTTTCTCCTTCTCACTTGGCCAG(+ATATAT)GTACCCCTAACTCCTGCTTGCAGAGGAATCC NGG 10 8 17 8 24 0 100 5 10
    240607022545_ndtd TCATTGGATTCCTCTGCAAGCAGGAGTTAGGG(+ATATAT)GTACCTGGCCAAGTGAGAAGGAGAAAAGTCC NGG 10 8 17 8 24 0 100 5 10
    240607022544_0k5e TATGAGACCTGACAGTTGAATGGGTGGCGTAG(+ATATAT)GATCCGTGAAAATTTTGGGCAGTCTTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607022543_fiho CGGGCTCCACAAGACTGCCCAAAATTTTCACG(+ATATAT)GATCCTACGCCACCCATTCAACTGTCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240607022541_oa5r CAAGAGGCCAGCCACTGAAGCCAGTGGCCTGG(+ATATAT)GTACCCAGGGGCCACTCCTTCCTCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240607022538_ryxz GGCCCAGTCTATGAGGAAGGAGTGGCCCCTGG(+ATATAT)GTACCCAGGCCACTGGCTTCAGTGGCTGGCC NGG 10 8 17 8 24 0 100 5 10