Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022520_3c5d GTCTACATCCTGGCTGGCTGGATGCCTACCTG(+ATATAT)GTACCCACTTGCCATGCAGAGGCCTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240607022519_sw8i ACCTCCCGGACAGGCCTCTGCATGGCAAGTGG(+ATATAT)GTACCAGGTAGGCATCCAGCCAGCCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240607022517_2q20 GAGCAGCTTCTAGCCTGGGTCACGAAGAGCAG(+ATATAT)GTACCCCTAGGAAGGCAGGAGAGTGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240607022515_rh8h GAACCCCTAGACACTCTCCTGCCTTCCTAGGG(+ATATAT)GTACCTGCTCTTCGTGACCCAGGCTAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607022513_bknk CTAACAACTGTCTGCAACTCCAGTCCCAAGGG(+ATATAT)GATCCAAAGGTCTCTTCTGGCCTCTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240607022512_3i7k ACCACATGTGCAGAGGCCAGAAGAGACCTTTG(+ATATAT)GATCCCCTTGGGACTGGAGTTGCAGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240607022510_czoh ATTTTTGCAAATAGATAAATTTAACAATCTTG(+ATATAT)GTACCCCACCTCAAAAGTGCAGTCACACAAA NGG 10 8 17 8 24 0 100 5 10
    240607022508_t1wd GCTGGTTTGTGTGACTGCACTTTTGAGGTGGG(+ATATAT)GTACCAAGATTGTTAAATTTATCTATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240607022507_azmu TAGGCTTCCTTCTCCTTGGAGGCCTGAGTTAG(+ATATAT)GTACCATGTCCACAACACTACAACTCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607022506_ds5x AGAGTCAAGAGAGTTGTAGTGTTGTGGACATG(+ATATAT)GTACCTAACTCAGGCCTCCAAGGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10