Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022608_smcd AGAAATTGCTGCACCAAGTCAATTGGCCGCTG(+ATATAT)GATCCGGATGTATCAGGCTGGAAACATGACT NGG 10 8 17 8 24 0 100 5 10
    240607022607_wmqj TCAGAAGTCATGTTTCCAGCCTGATACATCCG(+ATATAT)GATCCAGCGGCCAATTGACTTGGTGCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607022605_kb1b ATTCTGTAGATCTGGCTAGCCTCAAACTCAGG(+ATATAT)GATCCGCCCACCTCTGCCTCCCACGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607022603_73r2 TAATTTCAGCACGTGGGAGGCAGAGGTGGGCG(+ATATAT)GATCCCTGAGTTTGAGGCTAGCCAGATCTAC NGG 10 8 17 8 24 0 100 5 10
    240607022602_2jo5 GCTATATTCAGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTAAGATGGCTGTGAGCCACCACG NGG 10 8 17 8 24 0 100 5 10
    240607022601_lm1y AACCACGTGGTGGCTCACAGCCATCTTAATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240607022600_wv03 TGACCTAGTTAAATACAGGGTAGGAATGAGAG(+ATATAT)GATCCCTCACCAGATGGAACAATCAGACTGA NGG 10 8 17 8 24 0 100 5 10
    240607022558_ti8f ACGTTTCAGTCTGATTGTTCCATCTGGTGAGG(+ATATAT)GATCCTCTCATTCCTACCCTGTATTTAACTA NGG 10 8 17 8 24 0 100 5 10
    240607022557_lbbv CTGTTATTATTGAAGATCATTTCCACTCTACG(+ATATAT)GTACCACAGGACACTTAAGACTCTTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240607022555_jpzn TGGCTCTTGTGAAGAGTCTTAAGTGTCCTGTG(+ATATAT)GTACCGTAGAGTGGAAATGATCTTCAATAAT NGG 10 8 17 8 24 0 100 5 10