Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022537_9ex6 AAGGAACTTCTTAGATGGGCTTGTGATGTCAG(+ATATAT)GTACCCCCCCACCCTTACAGAGCGCAGACAT NGG 10 8 17 8 24 0 100 5 10
    240607022535_6mix GGCTCATGTCTGCGCTCTGTAAGGGTGGGGGG(+ATATAT)GTACCTGACATCACAAGCCCATCTAAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607022533_q0m3 AGATCCTGCTTCTCATGGGACTTCGATGAATG(+ATATAT)GATCCAGCCGCACAATCTACCCCTTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240607022532_ddld AGGGCACTGGGAAGGGGTAGATTGTGCGGCTG(+ATATAT)GATCCATTCATCGAAGTCCCATGAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607022530_tz5g CATCAGGAGCGACACCAAACAGAGTGCGTGGG(+ATATAT)GATCCCCAATTGGCCCAGTGTCCCCCGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607022528_lvvl AGGAAGGGCCGGGGGACACTGGGCCAATTGGG(+ATATAT)GATCCCCACGCACTCTGTTTGGTGTCGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607022527_91bz AGGCCCCTCACAACCCTTCTGCTCCAAGAGGG(+ATATAT)GATCCAGCAGGTGCCTCTGCCCAGGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240607022525_v16o GGAAGCCAGGCCCTGGGCAGAGGCACCTGCTG(+ATATAT)GATCCCCTCTTGGAGCAGAAGGGTTGTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607022524_5sv5 AACCTGATCAGAGCCTCCTTTCCTTGTTTTGG(+ATATAT)GATCCTGAATGGGCAAGAGTCTGTCCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607022522_hmwz GGCCCACAGGGGACAGACTCTTGCCCATTCAG(+ATATAT)GATCCCAAAACAAGGAAAGGAGGCTCTGATC NGG 10 8 17 8 24 0 100 5 10