Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022432_18pz CACACACCTGCTTCCTGGGTCCTATGGACAGG(+ATATAT)GTACCCACCATACCCTTCCTATCCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607022430_5c3m CCTGGGAGGGAGGGATAGGAAGGGTATGGTGG(+ATATAT)GTACCCTGTCCATAGGACCCAGGAAGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607022429_ezdy GGTTGGCTTCGCTGTTTTCCGGTTCCGTTCCG(+ATATAT)GATCCAGCCTAGGCCTCACACACACCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607022427_6z49 GCCCCTGCCCGGTGTGTGTGAGGCCTAGGCTG(+ATATAT)GATCCGGAACGGAACCGGAAAACAGCGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607022425_08x5 CCAGCCTCTTGAAAGTTAGGATGGGGTGGGAG(+ATATAT)GATCCCACTTTTCTTATTGGCTGGAGGACAA NGG 10 8 17 8 24 0 100 5 10
    240607022424_nv2s GCACATTGTCCTCCAGCCAATAAGAAAAGTGG(+ATATAT)GATCCTCCCACCCCATCCTAACTTTCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607022422_9tpv TCTTACTCCTGAGTATCCCAGGAGCTCAAAGG(+ATATAT)GTACCGGGTGGTGTGCAGCGGGGTTAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607022420_4i1x TGGCCCCTTCTAACCCCGCTGCACACCACCCG(+ATATAT)GTACCCTTTGAGCTCCTGGGATACTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607022418_8val GCTTCAGTGGAGGCCGGCCGGGGGAGGGAAGG(+ATATAT)GATCCTATATTTCCTGCTGACTCTTATTAGG NGG 10 8 17 8 24 0 100 5 10
    240607022417_1ie6 CTGACCCTAATAAGAGTCAGCAGGAAATATAG(+ATATAT)GATCCCTTCCCTCCCCCGGCCGGCCTCCACT NGG 10 8 17 8 24 0 100 5 10