Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022637_i69a CCCACACACACAATATTTTGCTACCTTTGTAG(+ATATAT)GTACCCTTCCTAAGAAGCTGAAATGTATGAC NGG 10 8 17 8 24 0 100 5 10
    240607022635_sylc ATGGGGTCATACATTTCAGCTTCTTAGGAAGG(+ATATAT)GTACCTACAAAGGTAGCAAAATATTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607022634_6qv7 GTACCCATGTACAATTATATTCACATTCTGAG(+ATATAT)GTACCGGAGATTGGGGTCTCAGAGTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240607022633_bbdu CCCAGATTTGTACTCTGAGACCCCAATCTCCG(+ATATAT)GTACCTCAGAATGTGAATATAATTGTACATG NGG 10 8 17 8 24 0 100 5 10
    240607022631_ax6s TGCTCTGAGGACCCGTATGACGGGTACTCTGG(+ATATAT)GTACCAGGCTACCAGGGACACACCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240607022629_zdjf AGAGCATCACTTGGTGTGTCCCTGGTAGCCTG(+ATATAT)GTACCCAGAGTACCCGTCATACGGGTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240607022628_1u3a GGTCACATCATCTTAACGTAAACATCAATAAG(+ATATAT)GTACCAACATTGTTAATGGAGGAAAAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240607022627_52qj TTTGGGTTGCTTTTCCTCCATTAACAATGTTG(+ATATAT)GTACCTTATTGATGTTTACGTTAAGATGATG NGG 10 8 17 8 24 0 100 5 10
    240607022626_9tb3 TGTCACGCAGAATGTGGTGTCCTGACTCAGTG(+ATATAT)GATCCCCGTGCCTGCCGGGATCTGTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240607022624_n5sq AAGTGGTGTTCACAGATCCCGGCAGGCACGGG(+ATATAT)GATCCACTGAGTCAGGACACCACATTCTGCG NGG 10 8 17 8 24 0 100 5 10