Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022753_qe79 CCTGGGAGGGAGCCTTTCCTGGCTGTTTCATG(+ATATAT)GATCCACCACAGGATGTTTTTTAAGCAATTT NGG 10 8 17 8 24 0 100 5 10
    240607022751_ybqq ATTGTAAATTGCTTAAAAAACATCCTGTGGTG(+ATATAT)GATCCATGAAACAGCCAGGAAAGGCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607022750_u8nn TAAGATGGGGAACACATGGGGGAGGGGCTGAG(+ATATAT)GATCCCTTTACCAAGGGCCTGAGGTCATACA NGG 10 8 17 8 24 0 100 5 10
    240607022749_9ox9 TTGAATGTATGACCTCAGGCCCTTGGTAAAGG(+ATATAT)GATCCTCAGCCCCTCCCCCATGTGTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607022746_o2ga CCCTGGTTGGGGTTCACCCGAGATGCCCAGGG(+ATATAT)GTACCGCCTCCAGCCTCAGGCCTGGAGCATT NGG 10 8 17 8 24 0 100 5 10
    240607022744_aoj0 GATGGAATGCTCCAGGCCTGAGGCTGGAGGCG(+ATATAT)GTACCCCTGGGCATCTCGGGTGAACCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240607022741_7l31 ACAGAAGTCACTGTCTTGCTGTCTAACCCAAG(+ATATAT)GATCCTATGTAGGGCCTCTCCAGAGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240607022740_n4kw ATGCCTTGTCTCTCTGGAGAGGCCCTACATAG(+ATATAT)GATCCTTGGGTTAGACAGCAAGACAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240607022739_3e8f CCTCTCCTGAGTCAGTTCTCTCTACCGTGTAG(+ATATAT)GTACCAGGGATCTAACTCGTGGCAGTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607022737_hjhe CGCTGTCCACACTGCCACGAGTTAGATCCCTG(+ATATAT)GTACCTACACGGTAGAGAGAACTGACTCAGG NGG 10 8 17 8 24 0 100 5 10